5-week old organoid (20x)

5-week old organoid (20x)

Green: PAX6 (nuclear marker for radial glial cells); White: Zo-1(marker for the apical end feet of radial glial cells (highlights the ventricular surface)); Magenta: CTIP2 (a nuclear marker for deep layer neurons)

Image: Ryan Hoffman
10 - week old organoid (10x)

10 - week old organoid (10x)

Green: PAX6 (nuclear marker for radial glial cells); Magenta: CTIP2 (nuclear marker for deep layer neurons); Red: SOX10 (nuclear marker the oligodendrocyte lineage); Light blue; SATB2 (nuclear marker for colossal projection neurons)

Image: Ryan Hoffman
10 - week old organoid (20x)

10 - week old organoid (20x)

Green: PAX (nuclear marker for radial glial cells); White: Zo-1 which (marker for the apical end feet of radial glial cells (highlights the ventricular surface)); Magenta: CTIP2 (nuclear marker for deep layer neurons)

Image: Ryan Hoffman
10- week old organoid (20x)

10- week old organoid (20x)

PAX6 (nuclear marker for radial glial cells); Magenta: CTIP2 (nuclear marker for deep layer neurons); Red: SOX10 (nuclear marker the oligodendrocyte lineage); Blue: AQP1 (apical marker of mature choroid plexus epithelia)

Image: Ryan Hoffman